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peg igm antibodies  (Bethyl)


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    Bethyl peg igm antibodies
    Peg Igm Antibodies, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peg igm antibodies/product/Bethyl
    Average 93 stars, based on 56 article reviews
    peg igm antibodies - by Bioz Stars, 2026-04
    93/100 stars

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    Quantification of serum anti-PEG antibody levels and results of a competitive ELISA using free mPEG-DSPE (experiment 1). (A) Serum anti-PEG IgG is shown to peak 7–14 d after the initial dose of house-MBs and decrease by the 28-d time point. (B) Serum anti-PEG IgM is shown to peak on day 7 for multiple and single dose groups. Anti-PEG IgM was found to decrease at subsequent time points. C and D) For those samples showing high anti-PEG IgM or anti-PEG IgG antibody expression, a competitive ELISA assay was performed to confirm PEG specificity. IgM and IgG binding to the PEG-coated ELISA plate was shown to decrease substantially when serum was incubated in the presence of free mPEG-DSPE (0.9–0.99 mg/ml). * p ≤ 0.05; ** p ≤ 0.01; **** p ≤ 0.0001. ELISA = enzyme-linked immunosorbent assay, Ig = immunoglobulin, DSPE-mPEG2000 = 1,2,-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy(polyethylene-glycol)-2000.

    Journal: Ultrasound in medicine & biology

    Article Title: Accelerated clearance of ultrasound contrast agents containing polyethylene glycol is associated with the generation of anti-polyethylene glycol antibodies

    doi: 10.1016/j.ultrasmedbio.2018.02.006

    Figure Lengend Snippet: Quantification of serum anti-PEG antibody levels and results of a competitive ELISA using free mPEG-DSPE (experiment 1). (A) Serum anti-PEG IgG is shown to peak 7–14 d after the initial dose of house-MBs and decrease by the 28-d time point. (B) Serum anti-PEG IgM is shown to peak on day 7 for multiple and single dose groups. Anti-PEG IgM was found to decrease at subsequent time points. C and D) For those samples showing high anti-PEG IgM or anti-PEG IgG antibody expression, a competitive ELISA assay was performed to confirm PEG specificity. IgM and IgG binding to the PEG-coated ELISA plate was shown to decrease substantially when serum was incubated in the presence of free mPEG-DSPE (0.9–0.99 mg/ml). * p ≤ 0.05; ** p ≤ 0.01; **** p ≤ 0.0001. ELISA = enzyme-linked immunosorbent assay, Ig = immunoglobulin, DSPE-mPEG2000 = 1,2,-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy(polyethylene-glycol)-2000.

    Article Snippet: Serum concentrations of anti-PEG IgM or anti-PEG IgG antibodies were analyzed separately using their respective enzyme-linked immunosorbent assay (ELISA) kits (Life Diagnostics, Inc., West Chester, PA, USA) performed according to the manufacturer’s protocol.

    Techniques: Competitive ELISA, Expressing, Binding Assay, Enzyme-linked Immunosorbent Assay, Incubation

    Enzyme-linked immunosorbent assay detection of serum anti-PEG IgM (A) and anti-PEG IgG (B) antibodies for animals repeatedly dosed with Definity or Optison over 30 d. PEG = polyethylene glycol; Ig = immunoglobulin

    Journal: Ultrasound in medicine & biology

    Article Title: Accelerated clearance of ultrasound contrast agents containing polyethylene glycol is associated with the generation of anti-polyethylene glycol antibodies

    doi: 10.1016/j.ultrasmedbio.2018.02.006

    Figure Lengend Snippet: Enzyme-linked immunosorbent assay detection of serum anti-PEG IgM (A) and anti-PEG IgG (B) antibodies for animals repeatedly dosed with Definity or Optison over 30 d. PEG = polyethylene glycol; Ig = immunoglobulin

    Article Snippet: Serum concentrations of anti-PEG IgM or anti-PEG IgG antibodies were analyzed separately using their respective enzyme-linked immunosorbent assay (ELISA) kits (Life Diagnostics, Inc., West Chester, PA, USA) performed according to the manufacturer’s protocol.

    Techniques: Enzyme-linked Immunosorbent Assay